FIGURE 3.

Conserved nucleotides are critical for THF binding by a representative folE motif RNA. The autoradiogram is the result of PAGE separation of in-line probing assay products for the wild-type (WT) M. loti 62 folE RNA and three mutant RNA constructs in the presence of 0 (–), 3, 30, and 300 µM THF (from left to right). Additional annotations are as described in the legend for Figure 2B. Positions of the mutations are based on the nucleotide numbering depicted in Figure 2A.