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The highly conserved glycine in the RNase III domains in KREPB6, KREPB7, and KREPB8 is essential for in vitro growth in BF (left panels) and PF (right panels) cells. Cumulative growth of BF and PF CN cells that constitutively express V5-tagged WT, or RNase III Glycine (G) to Arginine (R) or Valine (V) mutant versions of KREPB6, KREPB7, or KREPB8 and in which the tet-regulatable WT alleles were repressed. Data are representative of two independent experiments.
