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. 2019 Sep;25(9):1118–1129. doi: 10.1261/rna.071290.119

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© 2019 Shen et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 2. — Synthetic nucleic acids and cell lines used in this study. (A) Duplex RNA and single-stranded oligonucleotides. (B) Structure of chemical modifications. (C) Cell lines used in this study. (D) PCR analysis of the number of GAA repeats in F4259 iPSCs and NPCs. Analyses were conducted as described in Li et al. (2015). Somatic instability of the long repeats can be detected. p designates passage number. (HMNs) Human motor neurons, (NPCs) neuronal progenitor cells, (iPSC) induced pluripotent stem cells.