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. 2019 Nov;23(6):379–387. doi: 10.29252/ibj.23.6.379

Fig. 2.

Fig. 2

Enzyme digestion of plasmids. (A) Lane 1, 1 kb DNA ladder; lane 2, construct [pBSK (+) simple-Amp-nucleoprotein gene] digested with restriction enzymes of NcoI and BamHI; lane 3, undigested pBSK (+) simple-Amp; lane 4, linearized pCA4 vector; lane 5, undigested pCA4. (B) Lane 1, 1 kb DNA ladder; lane 2, plasmids extracted from clones after ligation reaction; Lanes 3-5, ligate digested with NcoI and BamHI