Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Washing steps of inclusion bodies. Lane 1, protein marker; lane 2, supernatant of second wash with lysis buffer; lane 3, supernatant of second wash with Tris EDTA Tween-20; lane 4, supernatant of second wash with Tris EDTA NaCl; lane 5, supernatant of second wash with Tris EDTA urea; lane 6, solubilized protein in solubilization buffer
