Figure 5.

H&E stain (100× magnification) showed inflammation cells (FGCs, lymphocytes, macrophages) and collagen deposition in the dermis of treated ischial area. There were various sizes of CaHA particles. Larger than 40-micron particles might be injected 1 month before the biopsy (1 m: 1 month) and a thin collagen layer was observed around the particle (1 m) without infiltration of the giant cell. There were many fibroblasts around the particles (1 m) (see Figure 6). Whereas dense deposits of thicker coarse-shape reinforced collagen fibers (C) were observed around smaller than 20-micron particles (7 m) that were injected 7 months before the biopsy. Around smaller than 20-micron particles (7 m), numerous FGCs and macrophages were also observed. FGCs ingested and cleaved CaHA particles (white arrows) into smaller sizes during chronic inflammation.