Figure 3.

a) An open pore current trace of a 7 nm diameter nanopore (25 nm thickness) in a conventional chamber. The open pore current is 6.85 ± 0.18 nA. b) An open pore current trace of the same pore, but assembled into the microfluidic chamber. The open pore current is 6.96 ± 0.19 nA. Both traces were measured using a 100 KHz bandwidth. c) Comparison of the power spectral density of the current in the conventional system and microfluidic system. The electrolyte solution is 1 M KCl 50 mM phosphate 5 mM EDTA. +500 mV bias toward trans chamber. Signals were filtered using the Axon 200B built-in Bessel filter at 100 KHz.