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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Mol Microbiol. 2019 Aug 8;112(4):1100–1115. doi: 10.1111/mmi.14349

Fig 5. Lytic transglycosylase activity is required for septal PG resolution.

Fig 5.

A) Expression of chromosomal Ptac: shyC, Ptac: shyA, Ptac: amiB nlpD envC, or Ptac: mltEE. coli was induced with 1 mM IPTG in a Δ2 LTG background, grown in LB at 37°C to ~OD600 0.6, and imaged on agarose pads. Analysis was conducted as described for Fig. 4A B) An overnight culture of Δ2 LTG Ptac: mltEE. coli grown in LB was spot-plated in 10-fold serial dilutions on low salt LB +/− 1mM IPTG and incubated for 18hrs at 30°C. All data are representative of at least two biological replicates.