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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Mol Carcinog. 2019 Jul 24;58(11):1974–1984. doi: 10.1002/mc.23089

Fig. 2. Effect of miR-214 overexpression on β-catenin protein.

Fig. 2.

(A) Overexpression of miR-214 evaluated by TaqMan qPCR analysis of metastatic melanoma cells transfected with 6nM mirVana mir-214 mimic at 24 h, 48 h and 72 h time points. miR-214 expression is shown relative to RNU48 expression. (B) TaqMan qPCR analysis of β-catenin mRNA in metastatic melanoma cells transfected with 6nM mirVana miR-214 mimic (M) or negative control oligo (C). Total RNA was extracted at 24 h, 48 h and 72 h post-transfection, analyzed by qRT-PCR and fold change in β-catenin mRNA over controls is shown. Data from 3 replicates are analyzed using unpaired Student’s t test where * indicates P values: *≤0.05; ** ≤0.01; *** ≤0.001 and **** ≤0.0001. (C) Western blot analysis for β-catenin protein expression in metastatic melanoma cells transfected with miR-214 mimic (M) or negative control (C). GAPDH levels show equal protein loading. Representative data from two independent experiments are shown.