Table 1.
Strain | % Av |
---|---|
FA1090 | 18.1 |
FA1090 G4 mutant | 0.2 |
FA1090 garP−10 | 0.1 |
FA1090 garP−35 | 6.4 |
FA1090 gar 72 NT | 16.9 |
FA1090 gar 32 NT | 17.6 |
rnhA+nicsPlac::rnhA 0 mm IPTG | 10.7 |
rnhA+nicsPlac::rnhA 0.005 mm IPTG | 13.9 |
rnhA+nicsPlac::rnhA 1 mm IPTG | 13.6 |
rnhA+nicsPlac::rnhA garP−10 0 IPTG | 0.2 |
This table shows the combined pilin av frequencies of each condition. All variable strains were tested in biological replicates. The individual pilin Av frequencies for each barcoded pool can be found in Supplemental Table 1 along with the number of reads analyzed for each pool.