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. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Immunity. 2019 Oct 3;51(4):655–670.e8. doi: 10.1016/j.immuni.2019.09.002

Figure 1.

Figure 1.

Recruited monocytes rapidly acquire expression of KC LDTFs followed by expression of a subset of KC-specific genes in KC-depleted livers

A. Experimental scheme: Clecf4-cre-tdTomato x Rosa26 LSL DTR +/− DT

B. Flow cytometry analysis of cell populations as a function of time following DT treatment.

C. MA plot of RNA-seq data comparing circulating monocytes and RLMs at 24 h. Data are from one or two experiments with n = 3–4 per group.

D. Genome-wide representation of DE genes in RLMs from 12h to 14 days in comparison to circulating monocytes and resident KCs. Data are from one or two experiments with n = 2–4 per group. DE genes are selected by DESeq2 (p-adj < 0.05).

E. PCA of 9568 detectable genes (at least 8 TPM in at least two samples) in circulating monocytes, recruited liver monocytes, RLMs and resident KCs.

F. Bar plots for expression of indicated genes. The significance symbols represent the p-adj from DESeq2 comparing to circulating monocytes respectively. ***p-adj < 0.001. Data are from one or two experiments with n = 2–4 per group. See also Figure S1.