Determining the optimal maximum ion injection time in context of ion trap scan rate and mass-to-charge ratio range. (A) MS/MS acquisition rates were calculated at specific mass-to-charge ratio scan widths at various ion trap scan rates (i.e., turbo, rapid, and normal). (B) The optimal maximum ion injection was determined by monitoring the MS/MS acquisition rate. For a 1 000 m/z scan range, the optimal maximum injection times were 13, 20, and 36 ms for turbo, rapid, and normal ion trap scan rates, respectively. (C) We expanded this analysis to mass-to-charge ratio scan widths between 200 m/z and 1900 m/z.