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. 2019 Oct 15;12:8501–8513. doi: 10.2147/OTT.S208263

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© 2019 Lv et al.

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FGFR1 is a direct target gene of miR-761 in OS cells. (A) The wild-type and mutant miR-761 binding sites in the 3ʹ-UTR of FGFR1. (B) Agomir-NC or agomir-761 was co-transfected with pmirGLO-WT-FGFR1-3ʹ-UTR and pmirGLO-MUT-FGFR1-3ʹ-UTR into U2OS and HOS cells. After 48 h culture, luciferase reporter assay was carried out to explore whether the 3ʹ-UTR of FGFR1 could be directly targeted by miR-761. *P<0.05 vs agomir-NC. (C, D) The mRNA and protein levels of FGFR1 in U2OS and HOS cells after gain of miR-761 were examined through RT-qPCR and Western blot analysis. *P<0.05 vs agomir-NC. (E) RT-qPCR was utilized to quantify FGFR1 mRNA expression in 61 pairs of OS tissues and corresponding adjacent normal tissues. *P<0.05 vs adjacent normal tissues. (F) The mRNA level of FGFR1 in OS cell lines (U2OS, MG-63, HOS, and SAOS-2) and normal human osteoblast cells (hFOB 1.19) was determined through RT-qPCR analysis. *P<0.05 vs hFOB1.19. (G) The expression correlation between miR-761 and FGFR1 mRNA in OS tissues was analyzed using Spearman’s correlation analysis. R²=0.3226, P<0.0001.