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. 2019 Oct 7;2019:8130481. doi: 10.1155/2019/8130481

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Copyright © 2019 I-Cheng Chen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of SCA3 patient-specific-induced pluripotent stem cells (SCA3-iPSCs). (a) iPSC colonies obtained from reprogramming of normal controls and patients' fibroblasts. (b) Genotyping of iPSCs and fibroblasts (HF). PCR analysis showed SCA3 patients carrying expanded CAG repeats (66~68 repeats). (c) Immunofluorescent staining for the stem cell markers alkaline phosphatase (AP), OCT4 and TRA-1-81 in control- and SCA3-iPSCs. (d) RT-PCR analysis of exogenous and endogenous pluripotency markers (OCT4, KLF4, SOX2, and c-MYC genes) from iPSC cell lines. (e) Karyotyping analysis of SCA3-iPSCs. (f) Teratoma formed after subcutaneously injecting SCA3-iPSCs into nude mice. HE staining of teratomas showed endoderm, mesoderm, and ectoderm lineages. Scale bar: 100 μm.