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. 2019 Oct 7;2019:8130481. doi: 10.1155/2019/8130481

Figure 4.

Figure 4

Impairment of the ubiquitin-proteasome system (UPS) in SCA3-iPSC-derived neurons treated with MG132. (a) Proteasome activity of iPSC-derived neurons treated with various concentrations of MG132 (0~5 μM). Proteasome activity of untreated neurons was normalized as 100%. (b) 1C2-positive polyQ aggregates in 2 μM MG132-treated iPSC-derived neurons were detected by the filter trap assay. (c) Representative images of iPSC-derived neurons stained with 1C2 (green) and anti-TUJ1 (red). The white arrows in the magnified picture indicate 1C2-positive aggregates colocalized with TUJ1. (d) Representative images of iPSC-derived neurons stained with a cellular ROS-detecting reagent (green). The white arrows in the magnified picture indicate ROS detected in TUJ1-positive neurons. Scale bar: 50 μm. Each experiment for each sample was performed in triplicate. p values: MG132-treated vs. untreated, ∗∗p < 0.01 and ∗∗∗p < 0.001; SCA3-1 vs. NC-1 or SCA3-2 vs. NC-2, &p < 0.05, &&p < 0.01, and &&&p < 0.001.