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. 2019 Oct 7;2019:8130481. doi: 10.1155/2019/8130481

Figure 5.

Figure 5

Elevated cytotoxicity and caspase 3 expression/activity in MG132-treated iPSC-derived neurons. (a) Lactic dehydrogenase (LDH) release in culture media was measured to evaluate cytotoxicity. LDH level of untreated neurons was normalized as 100%. (b) Western blot analysis of the cleaved caspase 3 level (normalized to TUJ1 as the internal control). Cleaved caspase 3 level of 2 μM MG132-treated NC-iPSC-derived neurons (NC-1 and NC-2) was set as 100%. (c) Caspase 3 activity assay of NC- and SCA3-iPSC-derived neurons. Caspase 3 activity of untreated neurons was normalized as 100%. Each experiment for each sample was performed in triplicate. p values: MG132-treated vs. untreated, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; SCA3-1 vs. NC-1 or SCA3-2 vs. NC-2, &p < 0.05, &&p < 0.01, and &&&p < 0.001.