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. 2019 Oct 7;2019:8130481. doi: 10.1155/2019/8130481

Figure 6.

Figure 6

NH037 and daidzein promoted proteasome function and ameliorated oxidative stress in MG132-treated SCA3-iPSC-derived neurons. (a) Proteasome activity of iPSC-derived neurons treated with 2 μM MG132 alone or MG132 together with 1 mg/ml NH037 or 50 μM daidzein. The proteasome activity of untreated neurons was normalized as 100%. (b) Western blot analysis of ubiquitin. TUJ1 was used as the internal control. Ubiquitinated protein level of MG132-treated NC-iPSC-derived neurons (NC-1 and NC-2) was set as 100%. (c) Lipid peroxidation malondialdehyde (MDA) assay was conducted to evaluate oxidative stress. MDA level of untreated neurons was normalized as 100%. (d) Representative images of SCA3 neurons staining with TUJ1 (red) and cellular ROS detecting reagent (green) after treatment with 2 μM MG132, 1 mg/ml NH037, or 50 μM daidzein. Scale bar: 50 μm. Each experiment for each sample was performed in triplicate. p values: MG132-treated vs. untreated, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; SCA3-1 vs. NC-1 or SCA3-2 vs. NC-2, &p < 0.05; MG132/NH037 vs. MG132 alone or MG132/daidzein vs. MG132 alone, #p < 0.05, ##p < 0.01, and ###p < 0.001.