Skip to main content
. 2019 Oct 7;2019:8130481. doi: 10.1155/2019/8130481

Figure 7.

Figure 7

NH037 and daidzein reduced cytotoxicity and caspase 3 expression/activity in MG132-treated SCA3-iPSC-derived neurons. (a) LDH assay in iPSC-derived neurons treated with 2 μM MG132 alone or MG132 and 1 mg/ml NH037/50 μM daidzein (untreated neurons were normalized as 100%). (b) Western blot analysis of cleaved caspase 3 (normalized to TUJ1 as the internal control) in iPSC-derived neurons treated with 2 μM MG132 alone or MG132 and 1 mg/ml NH037/50 μM daidzein. The levels of cleaved caspase 3 from MG132-treated NC-iPSC-derived neurons (NC-1 and NC-2) were set as 100%. (c) Caspase 3 activity in iPSC-derived neurons treated with 2 μM MG132 alone or MG132 and 1 mg/ml NH037/50 μM daidzein. Untreated neurons were normalized as 100%. Each experiment for each sample was performed in triplicate. p values: MG132-treated vs. untreated, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001; SCA3-1 vs. NC-1 or SCA3-2 vs. NC-2, &p < 0.05, &&p < 0.01, and &&&p < 0.001; MG132/NH037 vs. MG132 alone or MG132/daidzein vs. MG132 alone, #p < 0.05, ##p < 0.01.