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. 2019 Oct 8;2019:8982147. doi: 10.1155/2019/8982147

Figure 1.

Figure 1

Lycorine protects against LPS-induced bone loss in mice. (a) Representative images and relative intensities of the distal femurs from X-ray radiograms of mice treated with PBS (n = 5), LPS (5 mg/kg/week) (n = 5), or LPS+lycorine (2.5 mg/kg/d, n = 5; 4 mg/kg/d, n = 5; 6 mg/kg/d, n = 6) were measured using the ImageJ program. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with PBS-treated mice. #p < 0.05 and ###p < 0.001 compared with LPS-treated mice. Similar results were obtained in three independent experiments. (b) Representative μCT images of distal femora 1.0 mm from the growth plate of mice treated with PBS (n = 5), LPS (5 mg/kg/week) (n = 5), LPS+lycorine (6 mg/kg/d) (n = 6), or lycorine only (6 mg/kg/d) (n = 5). Mice were treated for 3 weeks beginning at the age of 10 weeks. (c) To examine TRAP-positive OCs in vivo, mouse femora were excised, cleaned with a soft tissue, and decalcified in EDTA. Representative histological sections of the distal femoral metaphysis of mice from each of the 4 groups were stained for TRAP to identify OCs (indicated by arrows head) to calculate OC.N/BS (OC number divided by total bone surface) and OC.S/BS (OC surface area divided by total bone surface area). Scale bar: 20 μm in the representative photos. ∗p < 0.05 and ∗∗p < 0.01 compared with PBS-treated mice. #p < 0.05 and ###p < 0.001 compared with LPS-treated mice. Differences between groups were analyzed by two-way ANOVA, followed by Bonferroni posttests to compare the effect of lycorine (OC.S/BS and OC.N/BS; p < 0.01) and the effect of LPS (OC.N/BS; p < 0.01) and interaction (OC.S/BS; p < 0.05). Similar results were obtained in three independent experiments.