Figure 5.
Microglia activation was determined by IF after modulation of the IL-17A/IL-17R pathway. (a) Representative image of the IF assay in the hippocampus at 12 hours post surgery (1 : 200). Specimens were stained with CD11b (green), Iba-1 (red), and DAPI (blue). Mean fluorescence intensity (MFI) of Iba-1 was shown in each image. (b) Summary data of the IF assay at 12 hours, 48 hours, and day 7 post CLP. ∗P < 0.05vs. sham control; #P < 0.05 (CLP+anti-IL17A ab vs. CLP+isotypeA; CLP+anti-IL17R ab vs. CLP+isotypeB).
