. 2019 Oct 8;2019:8530273. doi: 10.1155/2019/8530273

Table 1.

Experimental design of pig vaccination and challenge.

Experimental group^a	Inoculum	Immunization route	Vaccination days	Challenge
Experimental group^a	Inoculum	Immunization route	Vaccination days	Inoculum^e	Dpv
G1	Ad-blank^b	IM	0, 21	HNPJ	28
G2	rAd-PEDV-S^c	IM	0, 21	HNPJ	28
G3	Inactivated vaccine^d	IM	0, 21	HNPJ	28
G4	PBS	IM	0, 21	HNPJ	28

^aEach group contained five 4-week-old weaned piglets. ^b–dPigs in these three groups were vaccinated intramuscularly with 10¹⁰ PFU/mL Ad-blank, 10¹⁰ PFU/mL rAd-PEDV-S, and 2 mL of commercial PEDV inactivated vaccine (inactivated virus of the genotype GIIb strain; virus titer before inactivation ≥ 10⁷ TCID₅₀), respectively. ^eEach pig was challenged orally with a virus suspension containing 2 × 10⁵ TCID₅₀ highly virulent genotype GIIb PEDV strain CH/HNPJ/2017. Dpv: days post vaccination; IM: intramuscularly.