Table 2.
Summary of clinical scores and fecal viral shedding for the challenged piglets in each group.
| Dpi | Ad-blanka (n = 5) | rAd-PEDV-Sa (n = 5) | Inactivated vaccinea (n = 5) | PBSa (n = 5) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NP | CT | CS | NP | CT | CS | NP | CT | CS | NP | CT | CS | |
| 0 | 0/5 | — | 0 | 0/5 | — | 0 | 0/5 | — | 0 | 0/5 | — | 0 |
| 1 | 1/5 | 25.67 | 2 | 0/5 | — | 0 | 0/5 | — | 0 | 2/5 | 23.42–26.79 | 3 |
| 2 | 3/5 | 22.36–23.82 | 2‐3 | 0/5 | — | 0 | 0/5 | — | 0 | 5/5 | 22.73–25.18 | 3 |
| 3 | 5/5 | 21.58–24.12 | 2‐3 | 0/5 | — | 0 | 0/5 | — | 0 | |||
| 4 | 1/5 | 23.02 | 2 | 1/5 | 25.44 | 1 | ||||||
| 5 | 2/5 | 21.52–26.26 | 2 | 2/5 | 24.58–26.43 | 3 | ||||||
| 6 | 3/5 | 23.54–28.09 | 2‐3 | 2/5 | 23.69–26.87 | 2 | ||||||
| 7 | 3/5 | 24.27–25.59 | 2‐3 | 2/5 | 25.80–28.99 | 2‐3 | ||||||
aPigs were challenged orally with 2 mL of 1 × 105 TCID50 highly virulent genotype GIIb PEDV strain. Dpi: days post inoculation for pigs; NP: number of PEDV-positive piglets; CT: cycle threshold value—a value greater than 30 was considered negative or below the detection limit of real-time PCR; CS: clinical score for fecal consistency—0 = normal, 1 = pasty, 2 = semiliquid, and 3 = liquid; —: samples with no CT value (no PEDV RNA detected). The blocks without any entry indicate that the PEDV-positive piglets were euthanized after infection was confirmed by clinical symptoms and fecal viral shedding.