Fig. 1.

Analysis of TMZ-resistant cell lines and identification of resistance-associated genes. a&b Clonogenic assay of the parental and resistant U87MG cells (a, r#10 as the resistant clone) and A172 (b, r#6 as the resistant clone). Cells were treated for 3 days and cultured until Day 7 (*P < 0.05). c Flowchart of differentiating the mitochondria-related genes that significantly differed in TMZ-resistant cells. In U87MG- and A172-resistant cells, 26 overlapping mitochondria-related genes were identified. d Heatmap shows the significance of the 26 genes in the two resistant cell lines. e Validation of these 26 candidative mitochondrial genes in the TCGA-GBM dataset was analyzed. Only the 5 genes which expression was statistically significant were shown. f Kaplan-Meier curves of the TCGA databases from SurvExpress [18]. Each line refers to cases in which SOD2 gene expression was higher or lower than the median