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. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Biochem Biophys Res Commun. 2019 Sep 24;519(4):838–845. doi: 10.1016/j.bbrc.2019.09.075

Fig. 3.

Fig. 3.

Inhibition of eIF5A Hypusination Reduces TGFβ/Fibronectin-Induced Metastatic Dissemination. (A) Schematic of the chicken embryo chorioallantoic membrane (CAM) assay for quantitative detection of MCF10CA1h breast cancer metastasis. (B) Primary tumors were isolated and weighed to obtain tumor mass in eggs treated with GC7 or water control. (C) Lung and liver tissues were analyzed for metastatic dissemination of primary tumor cells via real-time PCR against human Alu repeats in relation to background chicken gapdh signal. Representative images of primary tumors, chicken lungs, and chicken livers after dissection and prior to analysis. *indicates p-value <0.05. (Scale bars: 0.5 cm for primary tumor, 1.0 cm for lung and liver).