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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Hepatol. 2019 Jul 8;71(5):960–969. doi: 10.1016/j.jhep.2019.06.019

Fig. 7. Hepatic Sirt6 overexpression protects against ALD in mice.

Fig. 7.

WT and Sirt6 transgenic (Tg_Sirt6) male mice were fed with a 6% ethanol (vol/vol) diet for 15 days plus a single binge (6 g/kg) on day 16. (A) Serum ALT measurements. (B, C) Hepatic TG and TC measurements. (D) H&E staining, IHC analysis of 4-HNE, ROS analysis by DHE and DCFDA dyes, and F4/80 and MPO staining. (E-G) Measurements of proinflammatory cytokines including TNF-α, IL-1β, and IL-6 in the liver tissues of WT and Sirt6 transgenic mice. Data are presented as means ± S.E.M (n = 4–6/group). #p < 0.05 and ##p < 0.01 for WT vs. Tg_Sirt6. H&E and IHC images were captured using a light microscope (200× magnification), and fluorescence images were obtained using a fluorescence microscope (200× magnification). Scale bars: 50 μm. ALT, alanine transaminase; DCFDA, dichlorofluorescin diacetate; DHE, dihydroethidium; 4-HNE, 4-hydroxynonenal; IHC, immunohistochemistry; ROS, reactive oxygen species; TC, total cholesterol; TG, triglyceride.