Table 2.

qPCR assays used to determine comparative levels of Htt transcripts

Assay	Primer/Probe	Sequence
Intron 1: 347F‐431R‐371P	Forward	5′‐TCCTCATCAGGCCTAAGAGCTGG‐3′
	Reverse	5′‐GAGACCTCCTAAAAGCATTATGTCATC‐3′
	Probe	5′‐AGTGCAGGACAGCGTGAGAGATGTG‐3′
Exon 1—Exon 2‐19F‐ex2R‐ex2P	Forward	5′‐AGGAACCGCTGCACCGA‐3′
	Reverse	5′‐CTGAGAGACTGTGCCACAATGTT‐3′
	Probe	5′‐AGAAAGACCGTGTGAATCATTGTCTAACAATATGTGA‐3′

The primers and probes for the TaqMan qPCR assay are listed.