Skip to main content
. Author manuscript; available in PMC: 2020 Oct 15.
Published in final edited form as: Immunity. 2019 Jul 25;51(4):682–695.e6. doi: 10.1016/j.immuni.2019.06.009

Figure 2. CGRP and its cognate receptor are induced in ILC2s during helminth infection.

Figure 2.

(A) Single-cell expression of receptors for NMU and CGRP in each cell cluster defined as in Figure 1C. (B) Kinetics of gene expression during N. brasiliensis infection in ILC2s for genes encoding receptors for NMU and CGRP using bulk mRNA-seq as in Figure 1B. (C-E) Calca expression in Th cells and ILCs using scRNA-seq (C-D) and confirmation by bulk mRNA-seq (E), as in A and B. (F) Immunohistochemical staining of lungs from Calca+/gfp mice 7 days after N. brasiliensis infection. The arrows depict ILC2s (CD3− KLRG1+) that express Calca (GFP+). The scale bar is 100 μm. (G) Calca expression in ILC2s from lung, mesentery and mesenteric LNs (mLNs) of Calca+/gfp mice before (0) and after N. brasiliensis infection (days 2 and 7). (H) Calca-driven expression of GFP in ILC2s from indicated tissues of Calca+/gfp mice, assessed by flow cytometry. (I) Accessibility of indicated loci in various cell types, as measured by ATAC-seq (data from Shih H et al. 2016: GSE77695). Data are from one experiment (A-E) or representative from three independent experiments with similar results (F-H). See also Figure S2.