Skip to main content
. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer Res. 2019 Aug 20;79(20):5394–5406. doi: 10.1158/0008-5472.CAN-19-0381

Figure 4.

Figure 4.

Treatment with immuno-NPs elicits a robust APC- and NK cell-driven immune response. Flow cytometry analysis of immune cells in the blood (a), tumor (c), and spleen (e) of 4T1 orthotopic mammary tumor-bearing mice 48 hr after treatment with immuno-nanoparticles (carrying 7 μg cdGMP and 6 μg MPLA) i.v. or immune checkpoint inhibitors (250 μg anti-PD1 and 100 μg anti-CTLA4 i.v.). Representative flow cytometry dot plots for significant immune cell subtypes are shown for each tissue type blood (b), tumor (d), and spleen (f). Treatment groups were made up of 6 mice, data are plotted as box and whiskers plots (5–95 percentile, + designates the mean) with statistics by 2-way ANOVA with Tukey’s post-test (* P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001).