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. Author manuscript; available in PMC: 2020 Oct 14.
Published in final edited form as: Cancer Cell. 2019 Sep 26;36(4):402–417.e13. doi: 10.1016/j.ccell.2019.08.006

Figure 1. EMT induction promotes a global decline in histone levels promoting H3.3-mediated gap filling and increasing chromatin accessibility.

Figure 1.

(A) Levels of various histones and Coomassie Blue stain of total histones in histone extracts of cells treated with TGFβ + TNFα (MCF-10A for 5 days, HCC1806 and A549 for 10 days) or MCF-10A expressing ERK2 D319N inducibly for 3 days; representative images (n = 4).

(B) Histone quantification by mass spectrometry in MCF-10A expressing ERK2 D319N inducibly for 3 days (n = 3). All values are expressed as mean ± SEM.

(C and D) Summary of genome-wide nucleosome occupancy showing distribution of detected peaks on chromosomes (All values are expressed as mean ± SEM) (C), and signal tracks for ZEB1 (D) determined by ATAC-seq in MCF-10A expressing ERK2 D319N inducibly for 3 days; arrows indicate the presence of novel peaks.

See also Figure S1 and Table S1.