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. 2019 Apr 21;12(6):1109–1125. doi: 10.1111/1751-7915.13410

Table 2.

Correlation between A. muciniphila and disease in animals

Subject Study type Study group Sample collection Sample detection Relevance conclusion
Catry et al. (2018) Nine‐week‐old male C57Bl/6J (WT) and Apoe−/− (KO) mice Interventional, fed an n‐3 polyunsaturated fatty acid (PUFA)‐depleted (DEF) diet for 12 weeks with or without inulin‐type fructans (ITFs) supplementation for the last 15 days

  1. WT DEF

  2. WT DEF ITF

  3. KO DEF

  4. KO DEF ITF

 Caecal content Illumina Sequencing of the 16S rRNA gene After prebiotic treatment of inulin‐type fructans, the endothelial dysfunction was improved in mice, and the abundance of A. muciniphila was increased
Zhu et al. (2017) Six‐week‐old male C57BL/6J mice Interventional, treated with fructo‐oligosaccharides and inulin for 6 weeks

  1. Blank control group

  2. High dose of FOS group

  3. Medium dose of FOS group

  4. Low dose of FOS group

  5. High dose of inulin group

  6. Medium dose of inulin group

  7. Low dose of inulin group

 Faeces 16S rRNA sequencing A. muciniphila became a dominant species in Verrucomicrobia phylum after treatment with fructo‐oligosaccharides and inulin. It played an important role on maintaining balance between mucin and short‐chain fatty acids
Singh et al. (2017) Male Swiss albino mice Interventional, HFD (58% fat kcal) for 12 weeks

  1. Normal pellet diet: n = 7–8

  2. HFD: n = 7–8

  3. Green tea extract: n = 7–8

  4. Isomalto‐oligosaccharide: n = 7–8

  5. Green tea extract + isomalto‐oligosaccharide: n = 7–8

 Caecal content 16S rRNA metagenomic sequencing A combination of green tea extract with isomalto‐oligosaccharide exerted beneficial effects on HFD‐induced alterations in mice and improved A. muciniphila abundances
Song et al. (2016) Male C57BL/6J mice Interventional, HFD plus HPBN of 200 mg/kg for 14 weeks

  1. Low‐fat diet: n = 24

  2. High‐fat diet: n = 24

  3. High‐fat diet + HPBN: n = 24

 Faeces 16S rRNA sequencing Red pitaya betacyanins protect from diet‐induced obesity and its related metabolic disorders, and increase the relative abundance of A. muciniphila
Schneeberger et al. (2015) Six‐week male C57BL/6 mice Interventional, HFD

  1. Normal diet: n = 24

  2. High‐fat diet: n = 24

 Caecal contents, collected at the time mice were sacrificed qPCR A. muciniphila abundance was reduced in obese mice induced by a high‐fat diet
Gomez‐Gallego et al. (2014) Two‐week BALB/c mice Interventional

  1. Breastfeeding group: n = 12

  2. Infant formula group: n = 12

  3. Infant formula group containing intermediate concentration polyamine: n = 12

  4. Infant formula group containing high concentration of polyamine: n = 12

 Oral, stomach, large and small intestine contents qPCR Compared with the infant formula group, A. muciniphila abundance was increased in the breastfeeding group
Baxter et al. (2014) 6–10 weeks male C57BL/6 mice Interventional, transplanted the faecal bacteria from three colorectal cancer patients and three healthy people to sterile mice (gavage)

  1. Faecal transplantation from healthy adults: n = 10

  2. Faecal transplantation from colorectal cancer: n = 10

  3. Control group: n = 5

 Transplanted human and mouse faeces, at day 0 and day 73 16S rRNA sequencing, Illumina sequencing The abundance of A. muciniphila in mice transplanted with faecal bacteria of colorectal cancer patients was higher than that of healthy adults
Hakansson et al. (2015) Wild female C57BL/6 mice Interventional, 4% DSS feeding for seven consecutive days

  1. Control group not treated with DSS: n = 10

  2. Test group treated with DSS: n = 10

 Colon and caecum contents, at day 7 16S rRNA sequencing, qPCR The A. muciniphila abundance in mice treated with 4% DSS was elevated compared to the untreated group
Zackular et al. (2013) 8–12 weeks male C57BL/6 mice Interventional, tumour‐inducing injection

  1. Control group: n = 10

  2. Induced tumour group: n = 9

 Faeces, collected daily during tumour‐injection 16S rRNA sequencing, qPCR A. muciniphila abundance was elevated in the faeces of tumour mice compared to that in healthy mice
Hansen et al. (2012) NOD mice (non‐obese diabetic mice) Interventional, 15–21 mice per group, vancomycin (83 mg kg−1 day−1)

  1. Adult group

  2. Newborn rat group

  3. Control group

 Faeces, collected at the time diagnosed as diabetes or blood glucose > 12 mM 16S rRNA sequencing, pyrosequencing A. muciniphila abundance was decreased in faeces of type 1 diabetic mice, and it was a protective strain of autoimmune diabetes
Berry et al. (2012) 6–8 weeks Wt mice and STAT1 −/− mice Interventional, the experimental group was given 2% DSS for 7 consecutive days, followed by drinking water for the next 3 days

  1. Experimental group

    Wt: n = 5

    STAT1−/−: n = 5

  2. Control group

    Wt: n = 5

    STAT1−/−: n = 5

 Colon and caecum contents, at day 10 16S rRNA sequencing, pyrosequencing The abundance of A. muciniphila in mice treated with 2% DSS was elevated compared to the control group
Sonoyama et al. (2010) Five‐week female BALB/c mice Interventional, ingesting 4 varieties of rice, then inducing allergic diarrhoea by immunization

  1. (Normal rice): n = 6

  2. (Wine rice): n = 6

  3. Glutinous rice): n = 6

  4. Yukihikari: n = 5

 Faeces, before immunization 16S rRNA sequencing, qPCR Compared with other groups, the abundance of A. muciniphila in the Yukihikari group was decreased, and the mice in this group were less likely to be induced to develop allergic diarrhoea
Sonoyama et al. (2009) 12‐week Syrian hamster Interventional, dietary intervention for 96 h

  1. Normal diet non‐hibernating mice: n = 6

  2. Fasted non‐hibernating mice: n = 6

  3. Hibernation mice: n = 6

 Caecal contents, at the end of the intervention qPCR A. muciniphila abundance was elevated in the fasted non‐hibernation mice compared to other groups

DSS, dextran sulfate sodium; FOS, fructo‐oligosaccharides; HFD, high‐fat diet; HPBN, hylocereus polyrhizus fruit betacyanins.