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. 2019 May 31;12(6):1249–1259. doi: 10.1111/1751-7915.13427

Figure 4.

Figure 4

Examples of successful integration at the locus sites. A. Detection of lipase activity on a tributyrin plate. When the colonies were surrounded by a clear zone, they were capable of lipase production. The GGA was used to transform a JMY195 strain, and the results were compared with those for the wild type, W29, and the lipase defective strain, JMY1212. B. Detection of glycogen synthase activity using Lugol's solution. When yellow wells were present, it indicated a defect in glycogen synthase, which is a characteristic of the Δgsy1 mutant strain (Bhutada et al., 2017). In this case, the JMY1212 strain was used to test the integration at gsy locus. These results were compared with those for the wild type, W29. C. Impact of integration site on gene expression levels using RedStarII as a reporter gene. The bars correspond to mean specific red fluorescence. The values correspond to the mean of four independent clones that have a correct locus integration. Error bars represent standard deviations.