Table 1.
The peak value of methanol metabolites (mM g−1 dry cell) and enzymatic activities (U g−1 dry cell) during three processes. (h‐sampling time)
| Fermentation strategy | Conventional strategy | Replacement strategy | Supplemented strategy |
|---|---|---|---|
| Metabolites | |||
| Formaldehyde (CH2O) | 19.1 (72 h) | 4.8 (72 h) | 1.9 (48 h) |
| Hydrogen peroxide (H2O2) | 14.9 (72 h) | 0.3 (48 h) | 0.4 (48 h) |
| Formate (CH2O2) | 0.014 (48 h) | 0.11 (72 h) | 0.011 (48 h) |
| Enzymes | |||
| Alcohol oxidase (AOX) | 20.3 (36 h) | 22.1 (24 h) | 21.6 (24 h) |
| Catalase (CAT) | 112.1 (36 h) | 12.6 (60 h) | 14.7 (48 h) |
| Formaldehyde dehydrogenase (FLD) | 20.2 (60 h) | 53.2 (60 h) | 126.9 (36 h) |
| Formate dehydrogenase (FDH) | 26.5 (36 h) | 127.2 (36 h) | 124.4 (48 h) |
| Malate dehydrogenase (MDH) | 8.6 (72 h) | 8.5 (24 h) | 8.1 (24 h) |
| Isocitrate dehydrogenase (IDH) | 2.3 (48 h) | 3.9 (36 h) | 3.9 (72 h) |
| α‐Ketoglutarate dehydrogenases (α‐KGDHs) | 1.4 (24 h) | 3.1 (48 h) | 3.1 (48 h) |