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The primer sequences of the upstream and downstream primers for these mRNA analyses (5′ to 3′).
| Genes | Upstream Primers | Downstream Primers |
|---|---|---|
| COL-1 | GTGCTAAAGGTGCCAATGGT | ACCAGGTTCACCGCTGTTAC |
| RUNX2 | CCCCACGACAACCGCACCAT | CACTCCGGCCCACAAATC |
| OSX ALP BSP OPN |
GCCAGAAGCTGTGAAACCTC CGAGATACAAGCACTCCCACTTC ATGGCCTGTGCTTTCTCAATG AGGAGGAGGCAGAGCACA |
GACAGCAGGGGACAGAAAAG CTGTTCAGCTCGTACTGCATGTC AGGATAAAAGTAGGCATGCTTG CTGGTATGGCACAGGTGATG |
