Figure 5.

Increased expression of HIF-1α in mouse CaPO₄ (A,D), mouse AngII (B,E), and human (C,F) aneurysms. Tissues from CaPO₄ mice (n = 5), AngII mice (n = 5) and PBS-only controls (n = 3), human AAA (n = 4), and human carotid plaques (n = 3) were enzymatically digested and processed to obtain single cell suspensions for flow cytometric analysis, as described in the Methods section. (A–C) depict the percentage of live cells expressing HIF-1α in aneurysmal and control tissues. (D–F) compare the percentage of live cells expressing HIF-1α between myeloid cells (CD11b⁺, TRAP⁻) and TPMs (CD11b⁺, TRAP⁺) from aneurysmal tissues. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. TPM, TRAP-positive macrophage. AAA, abdominal aortic aneurysm.