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. 2019 Oct 3;20(19):4905. doi: 10.3390/ijms20194905

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative fluorescent protein profiles using two-dimensional difference in gel electrophoresis (2D-DIGE) containing (A) a metabolically unhealthy obese (MUHO) sample labeled with Cy5, (B) a metabolically healthy obese (MHO) sample labeled with Cy3, and (C) a pooled internal control labeled with Cy2. Proteins were separated on IPG strip (pH 3–11) in the first dimension followed by 12.5% PAGE in the second dimension electrophoresis. Images were captured using a Typhoon 9400 Variable Mode. (D) An overlay of Cy3/Cy5 images is shown.