Table 2.
Primary structures and physicochemical properties of linear peptide 1 and its tryptic fragments (Figure 3).
| Peak Number | Retention Time (min) a | Amino Acid Sequence b | Theoretical Mass (Da) | Experimental Mass (Da) |
|---|---|---|---|---|
| 1 | 7.21 | MNYMVYFNFFACVLVPLLLMLGVYLYGRKKRRQRRR-amide | 4514.60 | 4514.00 |
| 2 | 7.46 | MNYMVYFNFFACVLVPLLLMLGVYLYGRKKRRQRR-carboxyl | 4359.40 | 4358.20 |
| 3 | 7.76 | MNYMVYFNFFACVLVPLLLMLGVYLYGRKKRRQR-carboxyl | 4203.20 | 4202.40 |
| 4 | 8.24 | MNYMVYFNFFACVLVPLLLMLGVYLYGRKKRR-carboxyl | 3918.89 | 3919.05 |
| 5 | 8.81 | MNYMVYFNFFACVLVPLLLMLGVYLYGRKKR-carboxyl | 3762.70 | 3763.35 |
| 6 | 9.57 | MNYMVYFNFFACVLVPLLLMLGVYLYGRKK-carboxyl | 3606.51 | 3606.45 |
| 7 | 10.90 | MNYMVYFNFFACVLVPLLLMLGVYLYGRK-carboxyl | 3478.34 | 3477.75 |
| 8 | 12.62 | MNYMVYFNFFACVLVPLLLMLGVYLYGR-carboxyl | 3350.16 | 3351.60 |
a Peptides were analyzed by HPLC with a 40–90% linear gradient of acetonitrile over 15 min. Identity was established by HPLC-mass spectrometry (MS) (details in Materials and Methods); b Residues in red correspond to the cell-penetrating sequence.