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Methylation-specific PCR primer design (a), qMSP (b) and agarose gel (c) for RUNX2, a key osteogenic regulator. There was no significant change in CEMP1 (d), IL 6 (e) and TNF α (f) methylation from different collection methods. The relationship of DNA methylation level and mRNA expression was correlated using a Spearman Rank correlation test (g–j). Each coloured dot Each coloured dot in Figure 6 (scatter graph; b,d–f) represents an individual participant.
