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. 2019 Sep 25;20(19):4751. doi: 10.3390/ijms20194751

Figure 3.

Figure 3

Intracellular calcium plays as an upstream of AMPK in EPA-mediated glucose uptake in skeletal muscle cells. (A) C2C12 cells pre-treated with fluo-3 AM for 30 min, and then treated with EPA. (B) Uptake of 2-DG in differentiated L6 myotubes pre-treated with STO-609 (5 µM) and incubated with EPA for 3 h. (C) Differentiated L6-GLUT4-myc cells were pre-treated with STO-609, and incubated with EPA for 3 h. (D) Uptake of 2-DG in differentiated L6 myotubes treated with BAPTA-AM (25 µM), and incubated with EPA for 3 h. (E) Western blot analysis of cell lysates from C2C12 cells pre-treated with 5 µM STO-609 for 30 min, and incubated with EPA. ** p < 0.01 compared to untreated cells. Results from three independently replicated experiments are presented.