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. 2019 Sep 25;20(19):4751. doi: 10.3390/ijms20194751

Figure 5.

Figure 5

AS160 is involved in EPA-induced GLUT4 expression in C2C12 myoblasts. (A) GLUT4 and β-actin transcripts measured by RT-qPCR from EPA-treated C2C12 cells. (B) Western blot analysis of GLUT4 and β-actin levels in cells stimulated with 50 μM EPA for the indicated times. The levels of β-actin are presented as a loading control. (C) Western blot analysis of cell lysates from cells transfected with AMPKα2 siRNA (100 nM) for 2 days and then treated with EPA. (D) p-AS160, AS160, and β-actin expression quantified using western blot analysis in cells transfected with AMPKα2 siRNA and treated with EPA. ** p < 0.01 compared to the basal condition. Results from three independently replicated experiments are presented.