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. 2019 Sep 21;20(19):4683. doi: 10.3390/ijms20194683

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bipartite DNA glycosylase domain in members of the DEMETER-LIKE (DML) family. (a) Schematic representation of ROS1 domains conserved among DML proteins. (b) Multiple sequence alignment of DML and HhH-GPD representative proteins. Critical residues for catalysis or DNA binding in ROS1 (REPRESSOR OF SILENCING 1) are indicated by asterisks (see text for more details). Names of organisms are abbreviated as follows: Ath, Arabidopsis thaliana; Nta, Nicotiana tabacum; Ta, Triticum aestivum; Osa, Oryza sativa; Zma, Zea mays; Bst, Bacillus stearothermophilus; Eco, Escherichia coli; Mth, Methanobacterium thermoautotrophicum; Mmu, Mus musculus; Hsa, Homo sapiens. Genbank accession numbers are: Ath ROS1: AAP37178; Ath DME: ABC61677; Nta ROS1: BAF52855; Tae DME: AEF38424; Osa DNG701: B8YIE8; Osa ROS1a: XP_015650531; Zma DME1: AQK70999; Bst EndoIII: 1P59; Eco EndoIII: P20625; Mth Mig: NP_039762; Eco MutY: NP_417436; Mmu MBD4: 1NGN; Hsa OGG1: O15527; Eco AlkA: P04395.