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. 2019 Sep 30;20(19):4867. doi: 10.3390/ijms20194867

Figure 2.

Figure 2

Ultrastructure and immunohistochemistry analyses by confocal microscopy. (A–C) Representative electron microscopy images of glomeruli and tubules of control and met-IPSC-derived embryoid bodies (EBs) showing podocyte-like cells (P), podocyte-like foot process (Pfp), mitochondria (M) and brush borders. The immunohistochemistry analysis in paraffin cuts reveals both PODXL and LTL positivity in embryoid bodies. Scale bar: 50 μm. (D–F) Representative electron microscopy images glomeruli and tubules of met-IPSC-derived kidney embryoid bodies revealing podocyte-like cells (P), podocyte-like foot process (Pfp), tight junctions, and typical brush border structures. Immunohistochemistry analysis in paraffin cuts by confocal imaging showed embryoid bodies structures co-expressing PODXL and LTL. Scale bar: 50 μm. As compared to control iPSC-derived embryoid bodies, the presence of glycogen granules(*) were noted (D). (G–H) Evaluation of functional analysis of proximal tubules structures in kidney embryoid bodies by using Dextran uptake assays. Embryoid bodies were incubated for 48 h in the presence of Dextran–Alexa 488 followed by analysis using wide-field microscopy. Dextran uptake was seen in normal (G) as well as in met-IPSC-derived embryoid bodies (H) with a more intense uptake in the latter structures (see text). Scale bar: 100 μm.