Figure 4.

MSD1 as a regulator of developmental signaling genes. (A) Yeast 1-Hybrid of MSD1-mediated activation of gene expression by binding to the MSD1 and MSD2 upstream promoter regions. (B) Teosinte branched/Cycloidea/PCF (TCP) binding motif enriched in the MSD1 DNA Affinity Purification sequencing (DAP-seq) peaks that are localized within 2000 bp of an annotated gene transcriptional start site (TSS). (C) RNA-seq data from msd1 showing downregulation across developing panicles stages in coordination with highlighted DAP-seq peaks localized near the transcriptional start sites (TSSs) of JA pathway genes. (D) Enriched DNA-binding motifs from all significant DAP-seq peak sequences, regardless of distance from an annotated gene TSS.