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. 2019 Oct 7;20(19):4946. doi: 10.3390/ijms20194946

Figure 1.

Figure 1

Gene expression of TAGLN in human bladder cells and tissues. (A) Total RNA from bladder smooth muscle cells (HBdSMC), fibroblast cells (HBdSF), normal epithelial cells (HBdEC), and carcinoma cell lines (RT4, HT1376, T24, and TSGH-8301) were extracted for RT-qPCR (± SE; n = 3) assays. (B) Bladder carcinoma cell lines (RT4, HT1376, T24, and TSGH-8301) were lysed, and TAGLN and β-actin were determined by immunoblotting. (C) HBdEC, HBdSMC, and HBdSF cells were lysed and α-SMA, UPK2, and GAPDH were determined by immunoblotting. (D) HBdEC and T24 cells were lysed and TAGLN and β-actin were determined by immunoblotting. Quantitative analysis of TAGLN expression in paired bladder cancerous and normal tissues was determined by RT-qPCR using the β-actin (E) or 18S (F) as the internal control. Box plot analysis was used to compare the TAGLN expressions in cancerous and normal bladder tissues (n = 25). ** represented the p < 0.01.