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. 2019 Oct 7;20(19):4946. doi: 10.3390/ijms20194946

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of TAGLN on the cell proliferation of bladder carcinoma cells. The expressions of TAGLN in T24-knockdown T24 (T24_shTAGLN) cells (A) and ectopic TAGLN-overexpressed HT1376 (HT-TAGLN) cells (B) in comparison to mock-transfected control cells (T24_shCOL and HT-DNA) were determined by immunoblot (top) and RT-qPCR (bottom) assays (± SE; n = 3). The proliferation abilities of T24_shCOL and T24_shTAGLN cells were determined by flow cytometry with Click-iT EdU flow cytometry assays (C) and EdU cell immunofluorescence staining (± SE; n = 4) (D). The proliferation abilities of HT-DNA and HT-TAGLN cells were determined by flow cytometry with (E) Click-iT EdU flow cytometry assays and (F) EdU cell immunofluorescence staining (± SE; n = 4). * represented the p < 0.05 and the ** represented the p < 0.01.

The effects of TAGLN on the cell proliferation of bladder carcinoma cells. The expressions of TAGLN in T24-knockdown T24 (T24_shTAGLN) cells (A) and ectopic TAGLN-overexpressed HT1376 (HT-TAGLN) cells (B) in comparison to mock-transfected control cells (T24_shCOL and HT-DNA) were determined by immunoblot (top) and RT-qPCR (bottom) assays (± SE; n = 3). The proliferation abilities of T24_shCOL and T24_shTAGLN cells were determined by flow cytometry with Click-iT EdU flow cytometry assays (C) and EdU cell immunofluorescence staining (± SE; n = 4) (D). The proliferation abilities of HT-DNA and HT-TAGLN cells were determined by flow cytometry with (E) Click-iT EdU flow cytometry assays and (F) EdU cell immunofluorescence staining (± SE; n = 4). * represented the p < 0.05 and the ** represented the p < 0.01.