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. 2019 Oct 7;20(19):4946. doi: 10.3390/ijms20194946

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Modulation of p53 and PTEN on TAGLN expression in bladder carcinoma cells. The mock-transfected HT1376 (HT-DNA) and p53-overexpressed HT1376 (HT-p53) cells were lysed, and then TAGLN, p53, and β-actin were determined by immunoblotting (A) and RT-qPCR (B) assays (± SE; n = 3). The mock-knockdown RT4 (RT4_shCOL) and p53-knockdown RT4 (RT4_shp53) cells were treated with camptothecin (1 μM) for 16 h. The cells were lysed, and then TAGLN, p53, and β-actin were determined by immunoblotting (C). (D) The reporter activity of TAGLN reporter vector cotrasnfected with various dosages of p53, as indicated in HT1376 cells. Data are expressed as the mean percentages ± SEs (n = 6) of luciferase activity relative to the mock-transfected group. The mock-transfected T24 (T24-DNA), PTEN-overexpressed T24 (T24-PTEN), mock-knockdown RT4 (RT4_shCOL), and PTEN-knockdown RT4 (RT4_shpPTEN) cells were lysed, and then TAGLN, PTEN, Akt, pAkt^S473, and β-actin were determined by immunoblotting (E). Expressions of PTEN and TAGLN in T24-DNA, T24-PTEN (F), RT4_shCOL, and RT4_shPTEN (G) cells were determined by RT-qPCR assays (± SE; n = 3). (H) The reporter activity of TAGLN reporter vector cotrasnfected with various dosages of PTEN, as indicated in HT1376 cells. Data are expressed as the mean percentages ± SEs (n = 6) of luciferase activity relative to the mock-transfected group. * represented the p < 0.05 and the ** represented the p < 0.01.

Modulation of p53 and PTEN on TAGLN expression in bladder carcinoma cells. The mock-transfected HT1376 (HT-DNA) and p53-overexpressed HT1376 (HT-p53) cells were lysed, and then TAGLN, p53, and β-actin were determined by immunoblotting (A) and RT-qPCR (B) assays (± SE; n = 3). The mock-knockdown RT4 (RT4_shCOL) and p53-knockdown RT4 (RT4_shp53) cells were treated with camptothecin (1 μM) for 16 h. The cells were lysed, and then TAGLN, p53, and β-actin were determined by immunoblotting (C). (D) The reporter activity of TAGLN reporter vector cotrasnfected with various dosages of p53, as indicated in HT1376 cells. Data are expressed as the mean percentages ± SEs (n = 6) of luciferase activity relative to the mock-transfected group. The mock-transfected T24 (T24-DNA), PTEN-overexpressed T24 (T24-PTEN), mock-knockdown RT4 (RT4_shCOL), and PTEN-knockdown RT4 (RT4_shpPTEN) cells were lysed, and then TAGLN, PTEN, Akt, pAkt^S473, and β-actin were determined by immunoblotting (E). Expressions of PTEN and TAGLN in T24-DNA, T24-PTEN (F), RT4_shCOL, and RT4_shPTEN (G) cells were determined by RT-qPCR assays (± SE; n = 3). (H) The reporter activity of TAGLN reporter vector cotrasnfected with various dosages of PTEN, as indicated in HT1376 cells. Data are expressed as the mean percentages ± SEs (n = 6) of luciferase activity relative to the mock-transfected group. * represented the p < 0.05 and the ** represented the p < 0.01.