Figure 2.

Effect of cardiac ischemia/reperfusion (I/R) injury on polyamine catabolism. Cardiac ischemia was induced in mice via the transient (30 min) occlusion of the left anterior descending artery. Cardiac tissue was harvested at 24 h post-surgery, processed for RNA extraction, and subjected to northern blot analysis (30 μg of RNA/lane). The results indicate that the expression of transcripts for spermine/spermidine N¹-acetyltransferase (Sat1) and spermine oxidase (Smox) genes increases following cardiac I/R injury. The northern blot shown here is a representative of four independent sample pairs. The 28S and 18S rRNA (bottom) show comparable RNA loading in sham and I/R samples.