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. 2019 Sep 25;20(19):4764. doi: 10.3390/ijms20194764

Figure 2.

Figure 2

Curcumin reduces activation of MAP-kinases and induces apoptosis and autophagy in neuroblastoma cells. (A) Protein lysates from IMR-32 and SH-SY5Y cells cultured in 10 or 20 µM curcumin respectively for 48 h (C = curcumin) were collected and subjected to Western blot analysis with anti-phospho-p38, anti-phospho-ERK 1/2, and anti-phospho-Akt antibodies. (B) The same protein lysates were blotted again and probed with anti-PARP for apoptosis (cl = cleaved PARP), and anti-Beclin-1 or anti-LC3 antibodies for autophagy (II = LC3-II). Untreated cells (U) were cultured with 0.1% DMSO. Protein levels were quantified by densitometry, normalized to the content of the loading control protein (each total protein or actin) and visualized by histograms. Data are representative of three independent experiments ± SD. (C) MYCN and c-MYC expression in IMR-32 and SH-SY5Y cell lines displayed by Western blot (** p < 0.01; *** p < 0.001).