Figure 4.

Curcumin treatment induces MYO18A up-regulation and TPX2 activation in neuroblastoma cells. (A) Protein lysates from IMR-32 and SH-SY5Y cells cultured in 10 or 20 µM curcumin respectively for 48 h (C = curcumin) and lysates from the same cells collected 48 h after curcumin depletion (pC = post-curcumin), were subjected to Western blot analysis with anti-GOLPH3 and anti-MYO18A antibodies. (B) The same lysates were subjected to Western blot analysis with anti-γH2AX and anti-TPX2 antibody. Untreated cells (U) were cultured with 0.1% DMSO. Protein levels were quantified by densitometry, normalized to the content of the loading control protein (total H2AX or actin) and visualized by histograms. Data are representative of three independent experiments ± SD (* p < 0.05; ** p < 0.01; *** p < 0.001).