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. 2019 Sep 25;20(19):4761. doi: 10.3390/ijms20194761

Figure 2.

Figure 2

Bactericidal effect of DAL-PEG-DK5 on S. aureus. (A) SYTO 9 and PI staining. Staphylococci were treated with DAL-PEG-DK5 (0.5–50 μg/mL) for 3 h and stained with SYTO 9 (all bacteria in the population) and PI (bacteria with damaged membrane). The fluorescence was measured (excitation at 485 nm and emission at 530 nm (SYTO9) and 630 nm (PI)). Mean ± SD n = 2. **** p < 0.0001; one-way ANOVA. (B) Time dependent influx of Sytox green into USA300. Staphylococci were incubated with 1 μM Sytox green for 15 min and then DAL-PEG-DK5 was added (5–100 μg/mL). Probes were incubated 60 min and fluorescence was measured at indicated time points (excitation at 485 nm and emission at 520 nm). The data shown is representative of three separate experiments performed in triplicate. Mean ± SD. ns – non-significant, ** p < 0.005; **** p < 0.0001; one-way ANOVA.