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. 2019 Sep 29;20(19):4845. doi: 10.3390/ijms20194845

Figure 3.

Figure 3

The efficacy of PEA-OXA on SIRT1 and UCP2 expression, the NAD+/NADH ratio, GSH, and SOD activity (induced by transient MCAo). A western blot analysis of SIRT1 showed basal expression that was significantly increased after MCAo. After PEA-OXA treatment, this expression was significantly upregulated, while after PEA administration, it increased less (A,B). Real-time PCR analysis of the SIRT1 gene displayed the same trend (E). UCP2 expression in vehicle-treated animals was significantly increased compared to the sham-operated animals. PEA-OXA administration was able to significantly restore the basal levels (C,D). PEA displayed less protection. The NAD+/NADH ratio was reduced by PEA-OXA treatment compared to the vehicle-treated animals (E). MCAo reduced GSH (G) and SOD (H) activity in vehicle-treated animals, while PEA-OXA administration restored the basal levels. A p-value of less than 0.05 was considered significant: * p < 0.05 versus sham; ° p < 0.05 versus vehicle; °° p < 0.01 versus vehicle; *** p < 0.001 versus sham; °°° p < 0.001 versus vehicle; # p < 0.05 versus PEA; ### p < 0.001 versus PEA.